Analysis of ergosterol for the detection of mold in soils by automated on-fiber derivatization headspace extraction-SPME-GC/MS.
Identifieur interne : 001A91 ( Main/Exploration ); précédent : 001A90; suivant : 001A92Analysis of ergosterol for the detection of mold in soils by automated on-fiber derivatization headspace extraction-SPME-GC/MS.
Auteurs : D-R Parkinson [Canada] ; J M Warren ; J. PawliszynSource :
- Analytica chimica acta [ 1873-4324 ] ; 2010.
English descriptors
- KwdEn :
- Automation, Ergosterol (analysis), Ergosterol (chemistry), Ergosterol (isolation & purification), Fungi (chemistry), Fungi (isolation & purification), Gas Chromatography-Mass Spectrometry, Limit of Detection, Soil Microbiology, Solid Phase Microextraction (economics), Solid Phase Microextraction (methods), Time Factors.
- MESH :
- chemical , analysis : Ergosterol.
- chemical , chemistry : Ergosterol.
- chemical , isolation & purification : Ergosterol.
- chemistry : Fungi.
- economics : Solid Phase Microextraction.
- isolation & purification : Fungi.
- methods : Solid Phase Microextraction.
- Automation, Gas Chromatography-Mass Spectrometry, Limit of Detection, Soil Microbiology, Time Factors.
Abstract
The estimate of ergosterol ([5,7,22-ergostatrien-3beta-ol] has been used by many to relate its concentration to the amount of mold in soils. This new method using on-fiber derivatization-solid phase microextraction-GC/MS method for the analysis of ergosterol presents a quick and straightforward method where low detection limits (1.5 ppb) and good limit of quantitation range (3 ppb to 90 ppm) can be achieved with careful control of analytical parameters. After saponification of real soil samples, sampling without extensive workup can be performed and analysis by a standard addition method can be utilized to deduce the original sample concentration of ergosterol. Peak area extraction analysis by MS SIM on selected characteristic fragment ions gives results with RSD < or = 2.2%.
DOI: 10.1016/j.aca.2009.12.027
PubMed: 20113733
Affiliations:
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Pawliszyn</name></author></analytic><series><title level="j">Analytica chimica acta</title><idno type="eISSN">1873-4324</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Automation</term><term>Ergosterol (analysis)</term><term>Ergosterol (chemistry)</term><term>Ergosterol (isolation & purification)</term><term>Fungi (chemistry)</term><term>Fungi (isolation & purification)</term><term>Gas Chromatography-Mass Spectrometry</term><term>Limit of Detection</term><term>Soil Microbiology</term><term>Solid Phase Microextraction (economics)</term><term>Solid Phase Microextraction (methods)</term><term>Time Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Ergosterol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ergosterol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Ergosterol</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="economics" xml:lang="en"><term>Solid Phase Microextraction</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Solid Phase Microextraction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Automation</term><term>Gas Chromatography-Mass Spectrometry</term><term>Limit of Detection</term><term>Soil Microbiology</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The estimate of ergosterol ([5,7,22-ergostatrien-3beta-ol] has been used by many to relate its concentration to the amount of mold in soils. This new method using on-fiber derivatization-solid phase microextraction-GC/MS method for the analysis of ergosterol presents a quick and straightforward method where low detection limits (1.5 ppb) and good limit of quantitation range (3 ppb to 90 ppm) can be achieved with careful control of analytical parameters. After saponification of real soil samples, sampling without extensive workup can be performed and analysis by a standard addition method can be utilized to deduce the original sample concentration of ergosterol. Peak area extraction analysis by MS SIM on selected characteristic fragment ions gives results with RSD < or = 2.2%.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Pawliszyn, J" sort="Pawliszyn, J" uniqKey="Pawliszyn J" first="J" last="Pawliszyn">J. Pawliszyn</name><name sortKey="Warren, J M" sort="Warren, J M" uniqKey="Warren J" first="J M" last="Warren">J M Warren</name></noCountry><country name="Canada"><noRegion><name sortKey="Parkinson, D R" sort="Parkinson, D R" uniqKey="Parkinson D" first="D-R" last="Parkinson">D-R Parkinson</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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